ZWINT antibody (AA 29-249)

Catalog No. ABIN4886776

This Rabbit Polyclonal antibody specifically detects ZWINT in WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ZWINT antibody (AA 29-249) (ABIN4886776)

Target: ZWINT (ZW10 Interactor (ZWINT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZWINT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ZWINT Antibody

Binding Specificity: AA 29-249

Purpose: Anti-ZWINT Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ZWINT Antibody Picoband® (ABIN4886776). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ZWINT recombinant protein (Position: Q29-Q249). Human ZWINT shares 58% and 57.1% amino acid (aa) sequence identity with mouse and rat ZWINT, respectively.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: ZWINT ZW10 interactor". 2. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology 6 (11): 1135-41. 3. van Vlijmen T, Vleugel M, Evers M, Mohammed S, Wulf PS, Heck AJ, Hoogenraad CC, van der Sluijs P (Aug 2008). "A unique residue in rab3c determines the interaction with novel binding protein Zwint-1". FEBS Letters 582 (19): 2838-42.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ZWINT

Target: ZWINT (ZW10 Interactor (ZWINT))

Alternative Name: ZWINT

Background:

Synonyms: ZW10 interactor,ZW10-interacting protein 1,Zwint-1,ZWINT,

Tissue Specificity: Detected in all tissues tested.

Background: ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene. This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molecular Weight: 31 kDa

Gene ID: 11130

UniProt: O95229