8-OHDG antibody

Catalog No. ABIN488679

Cited in 3+ publications. The Mouse Monoclonal anti-8-OHDG antibody (Clone 15A3) (ABIN488679) specifically detects 8-OHDG in ELISA, AC and IHC (fp).

Quick Overview for 8-OHDG antibody (ABIN488679)

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Host: Mouse

Clonality: Monoclonal

Conjugate: This 8-OHDG antibody is un-conjugated

Application: ELISA, Affinity Chromatography (AC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 15A3

Product Details anti-8-OHDG Antibody

Purpose: Anti-8-Hydroxyguanosine | DNA/RNA oxidative damage (clone 15A3)

Specificity: Recognizes markers of oxidative damage to DNA (8-hydroxy-2?-deoxyguanosine,8-hydroxyguanine and 8-hydroxyguanosine).

Cross-Reactivity (Details): Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Purification: Protein G purified

Immunogen: 8-hydroxy-guanosine-BSA and - casein conjugates

Isotype: IgG2a

Application Details

Application Notes: The optimal working dilution should be determined by the investigator

Comment:

protocol for immunostaining using this antibody can be found here

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Total IgG fraction. Protein G purified.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-8-OHDG antibody (ABIN488679)

Haigh, Drew: "Cavitation during the protein misfolding cyclic amplification (PMCA) method--The trigger for de novo prion generation?" in: Biochemical and biophysical research communications, Vol. 461, Issue 3, pp. 494-500, (2015) (PubMed).

Bobal, Otevrel, Poborilova, Vaverkova, Csollei, Babula: "Application of BY-2 cell model in evaluating an effect of newly prepared potential calcium channel blockers." in: Pakistan journal of pharmaceutical sciences, Vol. 28, Issue 4, pp. 1281-93, (2015) (PubMed).

Pilger, Rüdiger: "8-Hydroxy-2'-deoxyguanosine as a marker of oxidative DNA damage related to occupational and environmental exposures." in: International archives of occupational and environmental health, Vol. 80, Issue 1, pp. 1-15, (2006) (PubMed).

Target Details for 8-OHDG

Target: 8-OHDG (8-Hydroxyguanosine (8-OHDG))

Alternative Name: 8-Hydroxyguanosine

Target Type: Chemical

Background:

Background: Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.

Additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 0,65 mg/mL