EGLN3 antibody (Internal Region)

Catalog No. ABIN4889115

This anti-EGLN3 antibody is a Rabbit Polyclonal antibody detecting EGLN3 in WB, ELISA and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for EGLN3 antibody (Internal Region) (ABIN4889115)

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EGLN3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-EGLN3 Antibody

Binding Specificity: Internal Region

Specificity: Reacts with EGLN3 from human, mouse and rat

Purification: Purified (protein A)

Immunogen: Synthetic peptide of derived from internal part of human EGLN3.

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC1:100 - 1:1000 WB1:200 - 1:2000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M, sucrose 2 %

Storage: 4 °C/-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details for EGLN3

Target: EGLN3 (Egl-9 Family Hypoxia Inducible Factor 3 (EGLN3))

Alternative Name: EGLN3

Background: Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylation on the NODD site by EGLN3 appears to require prior hydroxylation on the CODD site.

Gene ID: 112399

UniProt: Q9H6Z9

Pathways: Positive Regulation of Endopeptidase Activity