N-epsilon-gamma-Glutamyl-Lysine Isopeptide antibody

Catalog No. ABIN4889369

The Mouse Monoclonal anti- antibody has been validated for ELISA, IHC and IF. It is suitable to detect in samples from All Species.

Quick Overview for N-epsilon-gamma-Glutamyl-Lysine Isopeptide antibody (ABIN4889369)

Target: N-epsilon-gamma-Glutamyl-Lysine Isopeptide

Reactivity: All Species

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 81D4

Product Details

Specificity: Reacts with Ne (g-L-glutamyl)-L-lysine

Cross-Reactivity (Details): Cross reacts in ELISA with N1,N4 bis (gPoly L-glutamyl) Putrescine and N1,N8 bis (gPoly L-glutamyl) Spermidine. No cross reaction with : - protein bound Ne acetyl lysine - protein bound N1 or N4 mono (gPoly L-glutamyl) Putrescine - protein bound N1 mono (gPoly L-glutamyl) Spermidine - protein bound Ne (g -L-glutamyl) lysine having a free alpha amine either on its glutamyl or lysyl moieties. Ability to fix complement : unknown

Purification: Purified

Immunogen: Nepsilon-(gamma-L-glutamyl)-L-lysine isopeptide

Isotype: IgM kappa

Application Details

Application Notes:

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only :
IHC(1:50 - 1:800)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: PO4 20 mM, NaCl 1M, sucrose 2 %

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized powder stable for a minimum of 2 years at -20°C. Store reconstituted antibodies at +4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 24 months

Target Details

Target: N-epsilon-gamma-Glutamyl-Lysine Isopeptide

Alternative Name: Nepsilon-(Gamma-L-Glutamyl)-L-Lysine Isopeptide

Background: Isopeptide bond are formed on lateral chains of proteins. Nε-(γ-L-glutamyl)-L-lysine isopeptide is generated after action of transglutaminase enzyme.