Nucleolin antibody (pThr76)

Catalog No. ABIN4889382

This anti-Nucleolin antibody is a Rabbit Polyclonal antibody detecting Nucleolin in WB, IF and IP. Suitable for Human and Mouse.

Quick Overview for Nucleolin antibody (pThr76) (ABIN4889382)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Nucleolin Antibody

Binding Specificity: pThr76

Specificity: Reacts with Nucleolin with phoshothreonine 76 (pT76) from human and mouse

Cross-Reactivity (Details): Do not cross react with phoshothreonine 84, 92 & 106 (pT76 and pT92). The detection of this P-NCL can be done during mitosis or in the centrosome during G1 phase,no signal in G2 phase.

Purification: Purified (protein A)

Immunogen: Synthetic peptide of human Nucleolin with a phoshothreonine at position 76

Application Details

Application Notes:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
WB1:100 - 1:1000 IF1:50 - 1:300

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization