Nucleolin antibody (pThr84)

Catalog No. ABIN4889383

This Rabbit Polyclonal antibody specifically detects Nucleolin in WB, IF and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for Nucleolin antibody (pThr84) (ABIN4889383)

Target: Nucleolin (NCL)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nucleolin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-Nucleolin Antibody

Binding Specificity: pThr84

Specificity: Reacts with Nucleolin with phoshothreonine 84 (pT84) from human and mouse

Cross-Reactivity (Details): Cross reacts with phoshothreonine 106 (pT106). Do not cross react with phoshothreonine 76 & 92 (pT76 and pT92). The detection of P-NCL can be done during mitosis or in G2 phase, no signal in G1S phase.

Purification: Purified (protein A)

Immunogen: Synthetic peptide of human Nucleolin with a phoshothreonine at position 84

Application Details

Application Notes:

Working dilution: Optimal dilutions should be determined by the end user.
The following are guidelines only:
- WB : 1/200 to 1/2000 - IF: 1/200 to 1/1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Must be reconstituted in distilled water.

Concentration: 1 mg/mL

Buffer: Tris 0,1M, glycine 0,1M

Storage: 4 °C/-20 °C

Storage Comment: Short term storage at+4°C. For extended periods store in aliquots at -20°C. Antibodies are guaranteed for 6 month from date of receipt.

Expiry Date: 6 months

Target Details for Nucleolin

Target: Nucleolin (NCL)

Alternative Name: Nucleolin

Background: Ucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.

Gene ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization