CCR4 antibody (Extracellular Domain)

Catalog No. ABIN490082

The Goat Polyclonal anti-CCR4 antibody has been validated for ELISA, WB, FACS, IHC and IHC (p). It is suitable to detect CCR4 in samples from Human and Monkey.

Quick Overview for CCR4 antibody (Extracellular Domain) (ABIN490082)

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This CCR4 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CCR4 Antibody

Binding Specificity: Extracellular Domain

Brand: IHC-plus™

Specificity: Peptide sequence is <50 % identical to other chemokine receptors in this region.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%) Gibbon, Panda (96%) Hamster, Dog, Horse (91%) Elephant, Rabbit (87%) Mouse (83%).

Purification: Immunoaffinity purified

Immunogen: 17SNYYLYESIPKPCTKEGIKAFGE39 corresponding to human CCR4 extracellular domain. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%), Gibbon, Panda (96%), Hamster, Dog, Horse (91%), Elephant, Rabbit (87%), Mouse (83%).

Application Details

Application Notes: Approved: ELISA (1:30000), Flo (1:12), IHC, IHC-P (5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM KHPO4, 140 mM NaCl with 1 mg/mL BSA and 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment: Short term: -20°C
Long term: -70°C
Avoid freeze-thaw cycles.

Target Details for CCR4

Target: CCR4 (Chemokine (C-C Motif) Receptor 4 (CCR4))

Alternative Name: CCR4

Background: Name/Gene ID: CCR4
Subfamily: Chemokine
Family: GPCR

Synonyms: CCR4, C-C chemokine receptor type 4, CD194, CD194 antigen, C-C CKR-4, Chemr13, CKR-4, CC chemokine receptor 4, CC-CKR-4, CCR-4, HGCN:14099, C-c chemokine receptor 4, Chemokine (C-C) receptor 4, CKR4, CMKBR4, K5-5

Gene ID: 1233

UniProt: P51679