Troponin I antibody

Catalog No. ABIN490086

The Mouse Monoclonal anti-Troponin I antibody (Clone 12F10) (ABIN490086) specifically detects Troponin I in WB, IHC and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for Troponin I antibody (ABIN490086)

Target: Troponin I (TNNI)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Troponin I antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 12F10

Product Details anti-Troponin I Antibody

Specificity: Cross-reactivity with cardiac TNI is less than 0.05 % .

Purification: Protein A purified

Immunogen: Mouse Skeletal muscle Troponin I.

Isotype: IgG2b

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze.

Target Details for Troponin I

Target: Troponin I (TNNI)

Alternative Name: TNNI / Troponin I Skeletal

Background: Name/Gene ID: TNNI

Synonyms: TNNI