Beclin 1 antibody (AA 110-190)

Catalog No. ABIN4901059

This Mouse Monoclonal antibody specifically detects Beclin 1 in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Beclin 1 antibody (AA 110-190) (ABIN4901059)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5C2

Product Details anti-Beclin 1 Antibody

Binding Specificity: AA 110-190

Purpose: Beclin-1 is a protein encoded by the ECN1 gene which is approximately 51,9 kDa. Beclin-1 is localised to the cytoplasm, Golgi apparatus and mitochondrion membrane. It is involved in the autophagy pathway, deubiquitination, metabolism of proteins, apelin signalling pathway and nanomaterial induced apoptosis. The beclin-1 protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which plays a role in multiple membrane trafficking pathways. PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. It also plays a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Beclin-1 is ubiquitously expressed. Mutations in the ECN1 gene can result in cervical cancer, breast cancer and Wolfram syndrome. STJ97761 was affinity-purified from rabbit. This antibody detects endogenous beclin-1.

Specificity: Beclin-1 Mouse Monoclonal Antibody (5C2) detects endogenous levels of BECN1

Characteristics: Mouse Monoclonal to Beclin-1.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: synthetic peptide derived from Beclin-1

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-2000
IHC 1:100-200

Comment:

Ubiquitous.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy