HSP27 antibody

Catalog No. ABIN4901717

The Mouse Monoclonal anti-HSP27 antibody has been validated for WB and ELISA. It is suitable to detect HSP27 in samples from Human and Monkey.

Quick Overview for HSP27 antibody (ABIN4901717)

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 7E5-1F9-D9

Product Details anti-HSP27 Antibody

Purpose: Mouse monoclonal to Hsp27.

Specificity: This antibody detects endogenous levels of Hsp27 and does not cross-react with related proteins.

Characteristics: Mouse Monoclonal to Hsp27.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Purified recombinant human Hsp27 protein fragments expressed in E.coli.

Isotype: IgG1

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for HSP27

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: Hsp27

Molecular Weight: 27kDa

Gene ID: 3315

UniProt: P04792

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling