CD86 antibody

Catalog No. ABIN490181

The Rat Monoclonal anti-CD86 antibody has been validated for FACS, IHC, IP, IHC (fro), ICC, Func, BR and Neut. It is suitable to detect CD86 in samples from Mouse.

Quick Overview for CD86 antibody (ABIN490181)

Target: CD86

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func), Blocking Reagent (BR), Neutralization (Neut)

Clone: GL1

Product Details anti-CD86 Antibody

Specificity: The rat monoclonal antibody GL-1 reacts with CD86 (B7-2), a 70-80 kD type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Purification: Protein G purified

Endotoxin Level: Low Endotoxin

Immunogen: LPS-activated CBA/Cs mouse splenic B cells.

Type of Immunogen: Cells

Isotype: IgG2a

Application Details

Application Notes: Approved: Block, Flo, Func, ICC, IHC, IHC-Fr, IP, Neut

Usage: Flow Cytometry: Recommended dilution - 2 μg/mL. Positive control - mouse splenocytes. Functional Application: Blocking. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Azide-free PBS,  pH 7.4, 0.2 micron filter sterilized

Preservative: Azide free

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: Name/Gene ID: CD86

Synonyms: CD86, Activation B7-2 antigen, B7-2, B7.2, B70, CD86 Molecule, CTLA-4 counter-receptor B7.2, CD86 antigen, FUN-1, LAB72, BU63, CD28LG2, Ctla-4 counter-receptors b7.2

Gene ID: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation