MAP1LC3A antibody

Catalog No. ABIN4901877

This anti-MAP1LC3A antibody is a Mouse Monoclonal antibody detecting MAP1LC3A in WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for MAP1LC3A antibody (ABIN4901877)

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAP1LC3A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5G10

Product Details anti-MAP1LC3A Antibody

Purpose: LC3A is a protein encoded by the MAP1LC3A gene which is approximately 14,3 kD. LC3A is localised to the cytoplasm, cytoskeleton and autophagosome. It is involved in pathways such as the autophagy pathway, pink/parkin mediated mitophagy, ferroptosis, cellular senescence and pathways affecting insulin-like growth factor-Akt signalling. MAP1A and MAP1B fall under the LC3A protein. They are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3A is most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. Mutations in the LC3A gene may result in Nasu-Hakola disease , lacrimal gland adenocarcinoma and leber congenital amaurosis. STJ97755 was developed from clone 5G10. It was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous levels of LC3A.

Specificity: LC3A Mouse Monoclonal Antibody (5G10) detects endogenous levels of LC3A

Characteristics: Mouse Monoclonal to LC3A.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: synthetic peptide derived from LC3A

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-2000
IHC 1:100-200

Comment:

Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for MAP1LC3A

Target: MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Alternative Name: LC3A

Gene ID: 84557

UniProt: Q9H492

Pathways: Autophagy