OCT4 antibody

Catalog No. ABIN4902143

The Mouse Monoclonal anti-OCT4 antibody has been validated for WB and ELISA. It is suitable to detect OCT4 in samples from Mouse.

Quick Overview for OCT4 antibody (ABIN4902143)

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This OCT4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 3F5-F9-E5

Product Details anti-OCT4 Antibody

Purpose: Mouse monoclonal to POU5F1/OCT4.

Specificity: This antibody detects endogenous levels of POU5F1/OCT4 and does not cross-react with related proteins.

Characteristics: Mouse Monoclonal to POU5F1/OCT4.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.

Isotype: IgG2b

Application Details

Application Notes: WB 1:500-2000
ELISA 1:10000-20000

Comment:

Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for OCT4

Target: OCT4 (POU5F1) (POU Class 5 Homeobox 1 (POU5F1))

Alternative Name: POU5F1/OCT4

Molecular Weight: 45kDa

Gene ID: 5460

UniProt: Q01860

Pathways: Stem Cell Maintenance