Ubiquitin antibody

Catalog No. ABIN4902435

This anti-Ubiquitin antibody is a Mouse Monoclonal antibody detecting Ubiquitin in WB and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for Ubiquitin antibody (ABIN4902435)

Target: Ubiquitin (Ub)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5F1

Product Details anti-Ubiquitin Antibody

Purpose: Ubiquitin is a protein encoded by the UBB gene which is approximately 25,7 kDa. Ubiquitin is localised to the cytoplasm and nucleus. It is involved in CDK-mediated phosphorylation and removal of Cdc6, RET signalling, activated TLR4 signalling and signalling by Notch1. It is one of the most conserved proteins known and has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression and the stress response. Ubiquitin is expressed in the nervous system, blood, liver and pancreas. Mutations in the UBB gene may result in a cleft palate. STJ97742 was developed from clone 5F1 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous levels of ubiquitin.

Specificity: Ubiquitin Mouse Monoclonal Antibody (5F1) detects endogenous levels of Ubiquitin

Characteristics: Mouse Monoclonal to Ubiquitin.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: synthetic peptide derived from Ubiquitin

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-2000
IHC 1:100-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C, and avoid repeat freeze-thaw cycles.

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway