CD3 antibody (PE)

Catalog No. ABIN490264

Product Details anti-CD3 Antibody (PE)

Target: CD3

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Purification: Protein A purified

Immunogen: Purified human CD3 proteins isolated from thymus.

Type of Immunogen: Purified protein

Clone: APA1-1

Isotype: IgG1

Application Details

Application Notes: Approved: Flo

Usage: The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 microgram/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 15 mM sodium azide, 0.2 % BSA, PBS

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze. Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store at 4°C. Do not freeze. Protect from light.

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway