B-Cell Linker antibody

Catalog No. ABIN4906623

This anti-B-Cell Linker antibody is a Mouse Monoclonal antibody detecting B-Cell Linker in WB. Suitable for Human.

Quick Overview for B-Cell Linker antibody (ABIN4906623)

Target: B-Cell Linker (BLNK)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 5C1

Product Details anti-B-Cell Linker Antibody

Cross-Reactivity: Human

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: This BLNK antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 150-178 amino acids from human BLNK.

Isotype: IgM

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background:

Synonyms: bca, AGM4, BASH, LY57, SLP65, BLNK-S, SLP-65, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, BLNK

Background: Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Gene ID: 29760

UniProt: Q8WV28

Pathways: BCR Signaling