GAPDH antibody (AA 1-335)

Catalog No. ABIN4909877

The Rabbit Polyclonal anti-GAPDH antibody has been validated for WB, ICC, IHC (p), IHC (fro), IF (cc) and IF (p). It is suitable to detect GAPDH in samples from Human, Mouse, Rat and Pig. There are 19+ publications available.

Quick Overview for GAPDH antibody (AA 1-335) (ABIN4909877)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-GAPDH Antibody

Binding Specificity: AA 1-335

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified by Protein A.

Immunogen: Recombinant full length human GAPDH

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GAPDH antibody (ABIN4909877)

Jia, Wang, Wang, Wei, Li, Wu, Hong: "Calycosin alleviates allergic contact dermatitis by repairing epithelial tight junctions via down-regulating HIF-1α." in: Journal of cellular and molecular medicine, Vol. 22, Issue 9, pp. 4507-4521, (2019) (PubMed).

Yu, Zhao, Zhang, Xie, Nie, Shi: "Inhibition of microRNA-143-3p attenuates myocardial hypertrophy by inhibiting inflammatory response." in: Cell biology international, Vol. 42, Issue 11, pp. 1584-1593, (2018) (PubMed).

Liang, Liu, Wang, Shen, Mei, Li, Liu: "Itraconazole exerts its anti-melanoma effect by suppressing Hedgehog, Wnt, and PI3K/mTOR signaling pathways." in: Oncotarget, Vol. 8, Issue 17, pp. 28510-28525, (2018) (PubMed).

Mu, Hui, Shao, Li, Du, Lu, Ye, Li, Li, Xiao, Qiu, Zhang, Fan, Ren, Tao, Xiang: "Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/β-catenin signaling and is frequently methylated in breast cancer." in: Oncotarget, Vol. 8, Issue 24, pp. 39443-39459, (2018) (PubMed).

Yin, Jiang, Shi, Gui, Yu: "Expression and Clinical Significance of ILF2 in Gastric Cancer." in: Disease markers, Vol. 2017, pp. 4387081, (2018) (PubMed).

Wang, Zhou, Xiong, Yu, Wu, Li, Yao, Wu, Hua: "Irreversible electroporation-mediated shRNA knockdown of the HPV18 E6 gene suppresses cervical cancer growth in vitro and in vivo." in: Oncology letters, Vol. 14, Issue 2, pp. 1943-1949, (2017) (PubMed).

Lin, Geng, Zhao, Lin, Zhu, Tian: "MiR-21 Regulates TNF-α-Induced CD40 Expression via the SIRT1-NF-κB Pathway in Renal Inner Medullary Collecting Duct Cells." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 41, Issue 1, pp. 124-136, (2017) (PubMed).

Liu, Xiao: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." in: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, (2017) (PubMed).

Zhou, Mu, Gong, Lu, Zhao, He, Qin: "Brd4 inhibition attenuates unilateral ureteral obstruction-induced fibrosis by blocking TGF-β-mediated Nox4 expression." in: Redox biology, Vol. 11, pp. 390-402, (2017) (PubMed).

Zong, Li, Zeng, Fang, Zhao: "The Effects of Interleukin-17 (IL-17)-Related Inflammatory Cytokines and A20 Regulatory Proteins on Astrocytes in Spinal Cord Cultured In Vitro." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 38, Issue 3, pp. 1100-10, (2016) (PubMed).

Yang, Dai, Tan, Zhang, Meng, Ye, Wang, Wei, He, Chen: "Lrig1 is a positive prognostic marker in hepatocellular carcinoma." in: OncoTargets and therapy, Vol. 9, pp. 7071-7079, (2016) (PubMed).

Qiao, Chen, Dong, Ma, Zhao, Tang, Li: "Polydatin Attenuates H2O2-Induced Oxidative Stress via PKC Pathway." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 5139458, (2016) (PubMed).

Duan, Tu, Liu, Jiao: "Antidepressant effect of electroacupuncture regulates signal targeting in the brain and increases brain-derived neurotrophic factor levels." in: Neural regeneration research, Vol. 11, Issue 10, pp. 1595-1602, (2016) (PubMed).

Guo, Zhao, Chen, Hu, Wang, Zhang, Sun: "BRAF-activated long non-coding RNA contributes to colorectal cancer migration by inducing epithelial-mesenchymal transition." in: Oncology letters, Vol. 8, Issue 2, pp. 869-875, (2014) (PubMed).

Wang, Guo, Zhao, Chen, Wang, Hu, Sun: "BRAF-activated long non-coding RNA contributes to cell proliferation and activates autophagy in papillary thyroid carcinoma." in: Oncology letters, Vol. 8, Issue 5, pp. 1947-1952, (2014) (PubMed).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background:

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH, GAPD, CDABP0047, OK/SW-cl.12 bs-0755R

Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Gene ID: 2597

UniProt: P04406