KLF4 antibody

Catalog No. ABIN4911412

This Mouse Monoclonal antibody specifically detects KLF4 in WB and IF (cc). It exhibits reactivity toward Human.

Quick Overview for KLF4 antibody (ABIN4911412)

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KLF4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 5A1

Product Details anti-KLF4 Antibody

Cross-Reactivity: Human

Purification: Purified mouse monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: KLF4 recombinant protein is used to produce this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: KLF4

Background:

Synonyms: EZF, GKLF, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4

Background: Transcription factor, can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.

Gene ID: 9314

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance