P4HB antibody

Catalog No. ABIN4912894

This Mouse Monoclonal antibody specifically detects P4HB in WB. It exhibits reactivity toward Human.

Quick Overview for P4HB antibody (ABIN4912894)

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This P4HB antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 3C3

Product Details anti-P4HB Antibody

Cross-Reactivity: Human

Purification: Purified by Protein G.

Immunogen: This PDIA1 antibody is generated from a mouse immunized with a recombinant protein of human PDIA1.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for P4HB

Target: P4HB (Prolyl 4-Hydroxylase, beta Polypeptide (P4HB))

Alternative Name: PDIA1

Background:

Synonyms: DSI, GIT, PDI, PHDB, PDIA1, PO4DB, PO4HB, PROHB, CLCRP1, ERBA2L, P4Hbeta, Protein disulfide-isomerase, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55, P4HB

Background: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9, the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).

Gene ID: 5034

UniProt: P07237

Pathways: Maintenance of Protein Location, Cell RedoxHomeostasis, Lipid Metabolism