TBP antibody

Catalog No. ABIN4914538

This Mouse Monoclonal antibody specifically detects TBP in WB, IHC (p) and IF (cc). It exhibits reactivity toward Human, Mouse, Rat and Cynomolgus.

Quick Overview for TBP antibody (ABIN4914538)

Target: TBP (TATA Box Binding Protein (TBP))

Reactivity: Human, Mouse, Rat, Cynomolgus

Host: Mouse

Clonality: Monoclonal

Conjugate: This TBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Clone: 8C4

Product Details anti-TBP Antibody

Cross-Reactivity: Human, Mouse, Rat

Cross-Reactivity (Details): Cynomolgus Monkey

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: Purified His-tagged TBP protein was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for TBP

Target: TBP (TATA Box Binding Protein (TBP))

Alternative Name: TBP

Background:

Synonyms: HDL4, GTF2D, SCA17, TFIID, GTF2D1, TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TBP, TF2D

Background: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

Gene ID: 6908

UniProt: P20226

Pathways: WNT Signaling