TRAF2 antibody

Catalog No. ABIN4914798

The Mouse Monoclonal anti-TRAF2 antibody (Clone 4C4) (ABIN4914798) specifically detects TRAF2 in WB and IF. The antibody is reactive with Human samples.

Quick Overview for TRAF2 antibody (ABIN4914798)

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 4C4

Product Details anti-TRAF2 Antibody

Cross-Reactivity: Human

Purification: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000
IF()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for TRAF2

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Alternative Name: TRAF2

Background:

Synonyms: TRAP, TRAP3, MGC:45012, TNF receptor-associated factor 2, E3 ubiquitin-protein ligase TRAF2, Tumor necrosis factor type 2 receptor-associated protein 3, TRAF2

Background: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation, this does not depend on the TRAF2 RING-type zinc finger domain. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE.

Gene ID: 7186

UniProt: Q12933

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response, S100 Proteins