VRK1 antibody

Catalog No. ABIN4915154

The Mouse Monoclonal anti-VRK1 antibody is suitable to detect VRK1 in samples from Human. It has been validated for WB.

Quick Overview for VRK1 antibody (ABIN4915154)

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VRK1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 5C1

Product Details anti-VRK1 Antibody

Cross-Reactivity: Human

Purification: Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.

Immunogen: Purified His-tagged VRK1 protein was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: WB 1:300-5000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for VRK1

Target: VRK1 (Vaccinia Related Kinase 1 (VRK1))

Alternative Name: VRK1

Background:

Synonyms: PCH1, PCH1A, Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1

Background: Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity.

Gene ID: 7443

UniProt: Q99986