Endonuclease G antibody (AA 51-140)

Catalog No. ABIN492521

The Mouse Monoclonal anti-Endonuclease G antibody has been validated for WB and EIA. It is suitable to detect Endonuclease G in samples from Human, Rat and Mouse.

Quick Overview for Endonuclease G antibody (AA 51-140) (ABIN492521)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 7G1C10

Product Details anti-Endonuclease G Antibody

Binding Specificity: AA 51-140

Specificity: Recognizes Endonuclease G (EndoG)

Characteristics: Synonyms: Endo-G, Endonuclease G mitochondrial

Purification: Immunoaffinity Chromotography.

Immunogen: Recombinant protein corresponding to amino acids 51–140 of Human EndoG.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: EndoG antibody can be used for detection of EndoG at 2.5-5 μg/mL. Positive Controls: HepG2 cell lysate, EndoG Recombinant Protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: Endonuclease G

Background: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro.Synonyms: ENDOG

Gene ID: 2021

UniProt: Q14249

Pathways: Apoptosis