Endonuclease G antibody (Middle Region)

Catalog No. ABIN2784751

There is 1 publication for this product available. The Rabbit Polyclonal anti-Endonuclease G antibody is suitable to detect Endonuclease G in samples from Human, Rat, Mouse, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio) and Cow. It has been validated for WB.

Quick Overview for Endonuclease G antibody (Middle Region) (ABIN2784751)

Target: Endonuclease G (ENDOG)

Reactivity: Human, Rat, Mouse, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Endonuclease G antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Endonuclease G Antibody

Binding Specificity: Middle Region

Purpose: ENDOG Antibody - middle region

Sequence: YVMPNAPVDE AIPLERFLVP IESIERASGL LFVPNILARA GSLKAITAGS

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ENDOG. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ENDOG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52054-100UG

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-Endonuclease G antibody (ABIN2784751)

Guha, Bandyopadhyaya, Polumuri, Chumsri, Gade, Kalvakolanu, Ahmed et al.: "Nicotine promotes apoptosis resistance of breast cancer cells and enrichment of side population cells with cancer stem cell-like properties via a signaling cascade involving galectin-3, α9 nicotinic ..." in: Breast cancer research and treatment, Vol. 145, Issue 1, pp. 5-22, (2014) (PubMed).

Target Details for Endonuclease G

Target: Endonuclease G (ENDOG)

Alternative Name: ENDOG

Background:

Background Information: ENDOG cleaves DNA at double-stranded (DG)n.(DC)n and at single-stranded (DC)n tracts. In addition to deoxyribonuclease activities, ENDOG also has ribonuclease (RNase) and RNase H activities. ENDOG is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.

Gene Name: Endonuclease G

Alternative Symbols: FLJ27463

Protein Name: Endonuclease G, mitochondrial

Molecular Weight: 28kDa

Gene ID: 2021

NCBI Accession: NP_004426

UniProt: Q14249

Pathways: Apoptosis