RAPTOR antibody

Catalog No. ABIN492625

This Mouse Monoclonal antibody specifically detects RAPTOR in WB. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for RAPTOR antibody (ABIN492625)

Target: RAPTOR

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This RAPTOR antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1-00E-011

Product Details anti-RAPTOR Antibody

Specificity: Recognizes Raptor.

Characteristics: Synonyms: RPTOR, KIAA1303, Regulatory-associated protein of mTOR, P150 target of rapamycinTOR-scaffold protein

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin derived from Raptor Sequence.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated Hela cellsDescription: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human)Format: Lyophilized cell lysate from serum starved HeLa cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for RAPTOR

Target: RAPTOR

Alternative Name: RAPTOR

Background: The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473Synonyms: KIAA1303, P150 target of rapamycin TOR-scaffold protein, RPTOR, Regulatory-associated protein of mTOR

Gene ID: 57521

UniProt: Q8N122

Pathways: Warburg Effect