Iba1 antibody (C-Term)

Catalog No. ABIN492698

The Goat Polyclonal anti-Iba1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect Iba1 in samples from Human, Rat and Mouse.

Quick Overview for Iba1 antibody (C-Term) (ABIN492698)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Iba1 Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes Allograft Inflammatory Factor-1 (AIF1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Bovine, Goat, Hamster, Rabbit, Pig, Horse, MonkeySpecies reactivity (tested):Human and Rat

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide (C-TGPPAKKAISELP) from C-terminus of Human AIF1

Isotype: IgG

Application Details

Application Notes: ELISA: 1/128000. Western blot: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This antibody was validated for use in Immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by AlkalinePhosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody in aliquots at-20 °C.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1

Background: Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.Synonyms: AIF-1, Allograft inflammatory factor 1, IBA1, Ionized Calcium-Binding Adapter Molecule 1, Protein G1

Gene ID: 199

NCBI Accession: NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration