Recombinant Cadherin-16 antibody

Catalog No. ABIN4949254

The Rabbit Monoclonal anti-Cadherin-16 antibody has been validated for IF, FACS and IHC (p). It is suitable to detect Cadherin-16 in samples from Human, Mouse and Rat.

Quick Overview for Recombinant Cadherin-16 antibody (ABIN4949254)

Target: Cadherin-16 (CDH16)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cadherin-16 antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CDH16-1532R

Product Details anti-Cadherin-16 Antibody

Purification: Protein A affinity chromatography

Immunogen: Human protein was used as the immunogen for the recombinant Cadherin 16 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Cadherin 16 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Cadherin 16 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Cadherin-16

Target: Cadherin-16 (CDH16)

Alternative Name: Cadherin 16

Background: Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that CDH16 (kidney-specific cadherin, Cadherin 16) is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, CDH16 is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. CDH16 has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.