Recombinant Caldesmon HMW antibody

Catalog No. ABIN4949259

This Rabbit Monoclonal antibody specifically detects Caldesmon HMW in FACS, IF and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for Recombinant Caldesmon HMW antibody (ABIN4949259)

Target: Caldesmon HMW

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CALD1-1424R

Product Details

Purification: Protein A affinity chromatography

Immunogen: A full length human protein was used as the immunogen for the recombinant Caldesmon antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant Caldesmon antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Caldesmon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Caldesmon HMW

Alternative Name: Caldesmon / CAD HMW

Background: Caldesmon (CAD, CALD, CALD1) is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). [UniProt]