Recombinant Caldesmon HMW antibody

Catalog No. ABIN4949259

Product Details

Target: Caldesmon HMW

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein A affinity chromatography

Immunogen: A full length human protein was used as the immunogen for the recombinant Caldesmon antibody.

Clone: CALD1-1424R

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant Caldesmon antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Caldesmon antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Caldesmon HMW

Alternative Name: Caldesmon / CAD HMW

Background: Caldesmon (CAD, CALD, CALD1) is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). [UniProt]