The Rabbit Polyclonal anti-CD235a/GYPA antibody has been validated for FACS, IF and IHC (p). It is suitable to detect CD235a/GYPA in samples from Human.
GYPA
Reactivity: Human
WB, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal dilution of the Glycophorin A antibody should be determined by the researcher.\. FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Preservative
Azide free
Storage
4 °C,-20 °C
Storage Comment
Store the Glycophorin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Target
CD235a/GYPA (GYPA)
(Glycophorin A (GYPA))
Alternative Name
Glycophorin A
Background
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175), binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]