Recombinant CD235a/GYPA antibody

Catalog No. ABIN4949344

The Rabbit Monoclonal anti-CD235a/GYPA antibody has been validated for FACS, IF and IHC (p). It is suitable to detect CD235a/GYPA in samples from Human.

Quick Overview for Recombinant CD235a/GYPA antibody (ABIN4949344)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GYPA-1725R

Product Details anti-CD235a/GYPA Antibody

Purification: Protein A affinity chromatography

Immunogen: Recombinant human GPA protein was used as the immunogen for the recombinant Glycophorin A antibody.

Isotype: IgG kappa

Application Details

Application Notes: Optimal dilution of the recombinant Glycophorin A antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Glycophorin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: Glycophorin A

Background: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175), binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]

Pathways: Maintenance of Protein Location