Recombinant MLANA antibody

Catalog No. ABIN4949359

The Rabbit Monoclonal anti-MLANA antibody has been validated for WB, IF, FACS and IHC (p). It is suitable to detect MLANA in samples from Human, Mouse and Rat.

Quick Overview for Recombinant MLANA antibody (ABIN4949359)

Target: MLANA (Melan A (MLANA))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MLANA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MLANA-1409R

Product Details anti-MLANA Antibody

Purification: Protein A affinity chromatography

Immunogen: Human protein was used as the immunogen for this recombinant MART-1 antibody.

Isotype: IgG kappa

Application Details

Application Notes: The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/10^6 cells,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MART-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for MLANA

Target: MLANA (Melan A (MLANA))

Alternative Name: Melan-A / MART-1

Background: MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]