p63 antibody

Catalog No. ABIN4949376

This Rabbit Polyclonal antibody specifically detects p63 in IF, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for p63 antibody (ABIN4949376)

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p63 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-p63 Antibody

Purification: Protein A affinity chromatography

Immunogen: Recombinant partial human p53 protein was used as the immunogen for this p63 antibody.

Isotype: IgG

Application Details

Application Notes: The optimal dilution of the p63 antibody for each application should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the p63 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for p63

Target: p63 (TP63) (Tumor Protein P63 (TP63))

Alternative Name: p63 / Tumor protein 63

Background: P63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands, however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90 % . Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80 %. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.