pan Keratin antibody

Catalog No. ABIN4949379

This Rabbit Polyclonal antibody specifically detects pan Keratin in IF, FACS, WB and IHC (p). It exhibits reactivity toward Human.

Quick Overview for pan Keratin antibody (ABIN4949379)

Target: pan Keratin (panKRT)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This pan Keratin antibody is un-conjugated

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-pan Keratin Antibody

Purification: Protein A affinity chromatography

Immunogen: Recombinant full-length human KRT76 and KRT77 proteins were used as the immunogen for this pan Cytokeratin antibody.

Isotype: IgG

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells in 0.1ml,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.25-0.5 μg/mL for 30 min at RT,Prediluted format: incubate for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the pan Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for pan Keratin

Target: pan Keratin (panKRT)

Alternative Name: Pan Cytokeratin, Acidic + Basic

Background: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody detects acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins: 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8), 56.5  kDa (CK10), 50  kDa (CK14), 50  kDa (CK15), 48  kDa (CK16), 40  kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. It is a broad spectrum anti pan-cytokeratin antibody, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It may be useful to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has high sensitivity in the recognition of epithelial cells and carcinomas.