Recombinant PD-1 antibody

Catalog No. ABIN4949389

The Rabbit Monoclonal anti-PD-1 antibody has been validated for FACS, IF and IHC (p). It is suitable to detect PD-1 in samples from Human.

Quick Overview for Recombinant PD-1 antibody (ABIN4949389)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: PDCD1-1410R

Product Details anti-PD-1 Antibody

Purification: Protein A affinity chromatography

Immunogen: A partial length human protein was used as the immunogen for the recombinant PD1 antibody.

Isotype: IgG kappa

Application Details

Application Notes: Titering of the recombinant PD1 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1),Immunofluorescence: 1-2 μg/mL,FACS: 0.5-1 μg/10e6 cells in 0.1ml

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant PD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PDCD1 / PD-1

Background: PDCD1 (Programmed cell death-1 protein), also called PD1 and CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. It is expressed on activated T-cells, B-cells, and myeloid cells. Anti-PDCD1 is a marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD1 is expressed by few B-cells, so may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma.

Pathways: Cancer Immune Checkpoints