beta Catenin antibody

Catalog No. ABIN4949465

This anti-beta Catenin antibody is a Mouse Monoclonal antibody detecting beta Catenin in WB, FACS, IF and IHC (p). Suitable for Human, Mouse and Rat.

Quick Overview for beta Catenin antibody (ABIN4949465)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: CTNNB1-1507

Product Details anti-beta Catenin Antibody

Purification: Protein G affinity chromatography

Immunogen: A partial human recombinant protein was used as the immunogen for the Beta Catenin antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the Beta Catenin antibody to be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL, IHC (FFPE): 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: Beta Catenin

Background: Beta-Catenin associates with the cytoplasmic portion of E-Cadherin, which is necessary for the function of E-Cadherin as an adhesion molecule. In normal tissues, beta-Catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-Catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immunostaining of beta-Catenin and E-Cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Pathways: Peptide Hormone Metabolism