CD3 epsilon antibody (AA 23-119)

Catalog No. ABIN4949514

This Mouse Monoclonal antibody specifically detects CD3 epsilon in FACS, WB, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for CD3 epsilon antibody (AA 23-119) (ABIN4949514)

Target: CD3 epsilon (CD3E)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: C3e-1308

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 23-119

Purification: Protein G affinity chromatography

Immunogen: Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the CD3e antibody should be determined by the researcher.\. WB: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. It is required for normal immune responses. This antibody is specific to the epsilon chain of CD3.

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway