CD81 antibody

Catalog No. ABIN4949542

The Mouse Monoclonal anti-CD81 antibody has been validated for FACS, WB, IF, Func and IHC (p). It is suitable to detect CD81 in samples from Human, Mouse and Rat.

Quick Overview for CD81 antibody (ABIN4949542)

Target: CD81

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Functional Studies (Func), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 1-3-3-22

Product Details anti-CD81 Antibody

Purification: Protein G affinity chromatography

Immunogen: A B-cell line derived from a Burkitt lymphoma was used as the immunogen for the CD81 antibody.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal dilution of the CD81 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,WB: 0.5-1 μg/mL,Functional Studies: order BSA free formulation,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the CD81 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for CD81

Target: CD81

Alternative Name: CD81

Background: This mAb recognizes a protein of 26  kDa, identified as CD81 (Workshop VI, Code CD81.1). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.

Pathways: Inositol Metabolic Process, Hepatitis C