EpCAM antibody (Extracellular Domain)

Catalog No. ABIN4949645

This anti-EpCAM antibody is a Mouse Monoclonal antibody detecting EpCAM in WB, FACS, IF, ELISA and IHC (p). Suitable for Human.

Quick Overview for EpCAM antibody (Extracellular Domain) (ABIN4949645)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: EGP40-1798

Product Details anti-EpCAM Antibody

Binding Specificity: AA 77-202, Extracellular Domain

Purification: Protein G affinity chromatography

Immunogen: A human recombinant partial protein corresponding to amino acids 77-202 (extracellular domain) was used as the immunogen for the Ep-CAM antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Titering of the Ep-CAM antibody may be required for optimal performance.\. ELISA: 2-4 μg/mL, order the BSA free formulation for coating,WB: 0.5-1 μg/mL,IF: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Ep-CAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM

Background: EGP40 is a 40-43  kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.