Interferon gamma antibody

Catalog No. ABIN4949769

This Mouse Monoclonal antibody specifically detects Interferon gamma in FACS, IF and IHC (p). It exhibits reactivity toward Human.

Quick Overview for Interferon gamma antibody (ABIN4949769)

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Interferon gamma antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: IFNG-466

Product Details anti-Interferon gamma Antibody

Purification: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for the IFN gamma antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: Optimal dilution of the IFN gamma antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the IFN gamma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Background: Recognizes a protein of 20-25  kDa, identified as human interferon. This mAb is specific to human IFNg and recognizes both recombinant and native human IFNg. T lymphocytes and NK cells mainly produce IFNg. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFNa/b (type I interferon).

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy