Thymidine Phosphorylase antibody

Catalog No. ABIN4949902

Product Details anti-Thymidine Phosphorylase Antibody

Target: Thymidine Phosphorylase (TYMP)

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thymidine Phosphorylase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Human recombinant full length protein was used as the immunogen for this Thymidine Phosphorylase antibody.

Clone: SPM322

Isotype: IgG1

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thymidine Phosphorylase antibody to be titered up or down for optimal performance.\. IP: 1-2 μg/500 μg lysate,IHC (FFPE): 1-2 μg/mL for 30 min at RT,WB: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Thymidine Phosphorylase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Alternative Name: Thymidine Phosphorylase / PD-ECGF (TYMP Products)

Synonyms: ECGF antibody, ECGF1 antibody, MEDPS1 antibody, MNGIE antibody, MTDPS1 antibody, PDECGF antibody, TP antibody, hPD-ECGF antibody, 2900072D10Rik antibody, Ecgf1 antibody, PD-ECGF antibody, Pdgfec antibody, typh antibody, thymidine phosphorylase antibody, thymidine phosphorylase DeoA antibody, AMP phosphorylase antibody, Thymidine phosphorylase antibody, TYMP antibody, Tymp antibody, deoA antibody, Dd586_0614 antibody, Kvar_4413 antibody, NMAG_RS19330 antibody, Snov_1187 antibody, Fbal_2856 antibody, Ftrac_2071 antibody, Intca_1216 antibody, ARCVE_RS10415 antibody, Sphch_4174 antibody, Sinme_3461 antibody, METOK_RS06355 antibody, Mesop_1831 antibody, typh antibody

Background: Recognizes a 485 amino acid protein (55 kDa monomer / 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), also called Thymidine Phosphorylase (TP, Tymp) or Gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2