TIMP2 antibody (AA 56-66)

Catalog No. ABIN4949915

This anti-TIMP2 antibody is a Mouse Monoclonal antibody detecting TIMP2 in WB, IF and IHC (p). Suitable for Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio) and Guinea Pig.

Quick Overview for TIMP2 antibody (AA 56-66) (ABIN4949915)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivity: Human, Rat, Mouse, Cow, Rabbit, Zebrafish (Danio rerio), Guinea Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 3A4

Product Details anti-TIMP2 Antibody

Binding Specificity: AA 56-66

Purification: Protein G affinity chromatography

Immunogen: Amino acids 56-66 (DSGNDIYGNPI) from the human protein were used as the immunogen for this TIMP-2 antibody.

Isotype: IgG2a kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP-2 antibody to be titered up or down for optimal performance.\. IHC (FFPE): 0.5-1 μg/mL for 30 min at RT,WB: 0.5-1 μg/mL,IF: 1-2 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the TIMP-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Alternative Name: TIMP2

Background: It recognizes a protein of 21  kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72  kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92  kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Pathways: cAMP Metabolic Process