EIF6 antibody (AA 66-210)

Catalog No. ABIN4950900

This Rabbit Polyclonal antibody specifically detects EIF6 in WB, IHC (p) and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EIF6 antibody (AA 66-210) (ABIN4950900)

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-EIF6 Antibody

Binding Specificity: AA 66-210

Purification: Antigen affinity

Immunogen: Amino acids 66-210 of human EIF6/EIF3A were used as the immunogen for the EIF3A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the EIF3A antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,ICC (Paraffin): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the EIF3A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for EIF6

Target: EIF6 (Eukaryotic Translation Initiation Factor 6 (EIF6))

Alternative Name: EIF6 / EIF3A

Background: EIF6 (Eukaryotic Translation Initiation Factor 6), also called EIF3A or ITGB4BP, is a human gene. By fluorescence in situ hybridization, Sanvito et al. (1998) mapped the ITGB4BP gene to 20q11.2. Ceci et al. (2003) demonstrated that the ribosomal 60S subunit is activated by release of EIF6. In the cytoplasm, EIF6 is bound to free 60S but not to 80S subunits. Furthermore, EIF6 interacts in the cytoplasm with RACK1, a receptor for activated protein kinase C. Gandin et al. (2008) demonstrated that mammalian eIF6 is required for efficient initiation of translation in vivo. Eif6-null mouse embryos were lethal at preimplantation. Heterozygous mice had 50 % reduction of eIF6 levels in all tissues, and showed reduced mass of hepatic and adipose tissues due to a lower number of cells and to impaired G1/S cell cycle progression.

UniProt: P56537

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly