FOXP3 antibody (AA 283-311)

Catalog No. ABIN3030998

The Rabbit Polyclonal anti-FOXP3 antibody has been validated for WB, FACS, ELISA and IHC. It is suitable to detect FOXP3 in samples from Human and Mouse.

Quick Overview for FOXP3 antibody (AA 283-311) (ABIN3030998)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC)

Product Details anti-FOXP3 Antibody

Binding Specificity: AA 283-311

Cross-Reactivity (Details): Expected species reactivity: Primate

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 283-311 from the human protein was used as the immunogen for this FOXP3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the FOXP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at 4°C for up to one month. For long term, aliquot the FOXP3 antibody and store frozen at -20°C or colder.

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3

Background: Forkhead box P3 is a transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon-gamma (IFNG). [UniProt]

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation