PKC alpha antibody

Catalog No. ABIN4953283

The Mouse Monoclonal anti-PKC alpha antibody is suitable to detect PKC alpha in samples from Rat and Primate. It has been validated for WB.

Quick Overview for PKC alpha antibody (ABIN4953283)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reactivity: Rat, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 3G11-G11-G11

Product Details anti-PKC alpha Antibody

Cross-Reactivity (Details): Expected species reactivity: Human

Purification: Protein G affinity

Immunogen: A synthetic PKC alpha/aPKC peptide was used as the immunogen for the PKC alpha antibody.

Isotype: IgG2a

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % Glycerol and 0.03 % ProClin 300

Storage: -20 °C

Storage Comment: Store the PKC alpha antibody at -20°C.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA / PKC alpha

Background: This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling