Recombinant GAPDH antibody (C-Term)

Catalog No. ABIN4953315

This Rabbit Monoclonal antibody specifically detects GAPDH in WB, ELISA, IHC, IP, ICC and FACS. It exhibits reactivity toward All Species.

Quick Overview for Recombinant GAPDH antibody (C-Term) (ABIN4953315)

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Antibody Type: Recombinant Antibody

Reactivity: All Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GAPDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: RM114

Product Details anti-GAPDH Antibody

Binding Specificity: C-Term

Characteristics: This recombinant GAPDH antibody reacts to Glyceraldehyde 3-phosphate dehydrogenase.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A peptide corresponding to the C-terminus of GAPDH was used as the immunogen for this recombinant GAPDH antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Western Blot: 1:1000,Immunoprecipitation: 1:200,Chromatin IP (ChIP): 1:200,Immunocytochemistry: 1:200,Immunohistochemistry: 1:200 (1)

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant GAPDH antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: GAPDH

Background: This recombinant GAPDH antibody reacts to Glyceraldehyde 3-phosphate dehydrogenase.

Gene ID: 2597

UniProt: P04406