Recombinant Histone 3 antibody (2meLys14)
Quick Overview for Recombinant Histone 3 antibody (2meLys14) (ABIN4953324)
Target
See all Histone 3 (H3) AntibodiesAntibody Type
Reactivity
Host
Clonality
Conjugate
Application
Clone
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Binding Specificity
- 2meLys14
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Characteristics
- This recombinant H3K14me2 antibody reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated (K14me1) or trimethylated Lysine 14 (K14me3), or other methylation in Histone H3.
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Purification
- Protein A purified from animal origin-free supernatant
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Immunogen
- A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys14) was used as the immunogen for this recombinant H3K14me2 antibody.
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Isotype
- IgG
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Application Notes
- The stated application concentrations are suggested starting points. Titration of the recombinant H3K14me2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.25-1 μg/mL,ELISA: 0.2-1 μg/mL
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Restrictions
- For Research Use only
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Buffer
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store the recombinant H3K14me2 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- Histone 3 (H3) (Histone H3 (H3))
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Alternative Name
- Histone H3
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Background
- This recombinant H3K14me2 antibody reacts to Histone H3 dimethylated at Lysine 14 (K14me2). No cross reactivity with monomethylated (K14me1) or trimethylated Lysine 14 (K14me3), or other methylation in Histone H3.
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Gene ID
- 8350
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UniProt
- P84243
Target
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