Recombinant Histone 3 antibody (meLys79)

Catalog No. ABIN4953342

This Rabbit Monoclonal antibody specifically detects Histone 3 in WB, ELISA and ChIP. It exhibits reactivity toward All Species.

Quick Overview for Recombinant Histone 3 antibody (meLys79) (ABIN4953342)

Target: Histone 3 (H3) (Histone H3 (H3))

Antibody Type: Recombinant Antibody

Reactivity: All Species

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Clone: RM147

Product Details anti-Histone 3 Antibody

Binding Specificity: meLys79

Characteristics: This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: A monomethyl-peptide corresponding to Monomethyl-Histone H3 (Lys79) was used as the immunogen for this recombinant H3K79me1 antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant H3K79me1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.2-1 μg/mL,ChIP: 2-10 μg/mg of lysate,ELISA: 0.2-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant H3K79me1 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for Histone 3

Target: Histone 3 (H3) (Histone H3 (H3))

Alternative Name: Histone H3

Background: This recombinant H3K79me1 antibody reacts to Histone H3 monomethylated at Lysine 79 (K79me1). No cross reactivity with dimethylated (K79me2), trimethylated (K79me3) or other methylations of Histone H3.

Gene ID: 8350

UniProt: P84243