Recombinant IgE antibody

Catalog No. ABIN4953368

The Rabbit Monoclonal anti-IgE antibody (Clone RM122) (ABIN4953368) specifically detects IgE in ELISA, IHC, ICC and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant IgE antibody (ABIN4953368)

Target: IgE

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This IgE antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: RM122

Product Details anti-IgE Antibody

Characteristics: This recombinant Human IgE antibody reacts to hIgE. No cross reactivity with IgG, IgM, IgD, or IgA.

Purification: Protein A purified from animal origin-free supernatant

Immunogen: Human IgE was used as the immunogen for this recombinant Human IgE antibody.

Isotype: IgG

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the recombinant Human IgE antibody may be required due to differences in protocols and secondary/substrate sensitivity.

1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 10 ng/well - 100 ng/well (Capture), 0.01 - 0.1 μg/mL (Detection),Immunocytochemistry: 0.5-2 μg/mL,Immunohistochemistry: 0.5-2 μg/mL (1)

Restrictions: For Research Use only

Handling

Buffer: 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the recombinant Human IgE antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).

Target Details for IgE

Target: IgE

Background: This recombinant Human IgE antibody reacts to hIgE. No cross reactivity with IgG, IgM, IgD, or IgA.

Gene ID: 3497

UniProt: P01854