ATG5 antibody (AA 209-238)

Catalog No. ABIN3030035

The Rabbit Polyclonal anti-ATG5 antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect ATG5 in samples from Human and Mouse.

Quick Overview for ATG5 antibody (AA 209-238) (ABIN3030035)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-ATG5 Antibody

Binding Specificity: AA 209-238

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 209-238 from the human protein was used as the immunogen for this anti-ATG5 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the anti-ATG5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:25,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the anti-ATG5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy