Caspase 3 antibody (AA 60-90)

Catalog No. ABIN3030306

This anti-Caspase 3 antibody is a Rabbit Polyclonal antibody detecting Caspase 3 in WB, IHC, ELISA and FACS. Suitable for Human.

Quick Overview for Caspase 3 antibody (AA 60-90) (ABIN3030306)

Target: Caspase 3 (CASP3)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-Caspase 3 Antibody

Binding Specificity: AA 60-90

Purification: Purified

Immunogen: A portion of amino acids 60-90 from the human protein was used as the immunogen for this anti-Caspase-3 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the anti-Caspase-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the anti-Caspase-3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Caspase 3

Target: Caspase 3 (CASP3)

Alternative Name: Caspase-3

Background: CASP3 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

UniProt: P42574

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation